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| Registros recuperados: 237 | |
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| Allam, L; Ogwu, D; Agbede, R.I.S; Sackey, A.K.B. |
| A study was conducted in 24 gilts to determine the effect of Trypanosoma brucei infection on their reproductive efficiency. The infected gilts developed clinical trypanosomosis following a prepatent period of 2-3 days with 1.8 x 10⁶ trypanosomes per gilt. The clinical signs were observed intermittent fever, pale mucus membranes, short moist cough, moist rales, mucopurulent ocular discharges and hyperemia of the skin, reduced feed intake, and loss of body condition, recumbency, uncoordinated movements, posterior paresis and death of gilts. The cause of death in the pigs was pneumonia caused by Escherichia coli. Grossly, the lungs were severely congested and had undergone gray hepatization. Histopathologically, the lungs had thickened and congested alveolar... |
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Palavras-chave: Trypanosoma brucei; Immunosuppression; Escherichia coli; Pneumonia; Gilts. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3538 |
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| Ananias,M.; Yano,T.. |
| Sixty strains of Escherichia coli, isolated by hemoculture, from septicemic Brazilian patients were evaluated to determine their serogroup and invasivity to Vero cells. All 60 patients died within 2 days of hospitalization. Furthermore, the molecular study of the following extraintestinal pathogenic E. coli-associated virulence factor (VF) genes was performed by PCR: i) adhesins: type 1 fimbria (fimH), S fimbria (sfaD/E), P fimbria (papC and papG alleles) and afimbrial adhesin (afaB/C); ii) capsule K1/K5 (kpsMTII); iii) siderophores: aerobactin (iucD), yersiniabactin (fyuA) and salmochelin (iroN); iv) toxins hemolysin (hlyA), necrotizing cytotoxic factor type 1 (cnf1) and secreted autotransporter toxin (sat); v) miscellaneous: brain microvascular... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Escherichia coli; Sepsis; Serogroups; Virulence genotypes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001000008 |
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| Aragão,Annelize Zambon Barbosa; Teocchi,Marcelo Ananias; Fregolente,Maria Clara Duarte; Gatti,Maria Silvia Viccari; Pires,Alexandre Vaz; Yano,Tomomasa. |
| Twenty seven (48.2%) culture supernatants of 56 Escherichia coli isolated from diarrheic lamb feces (7 to 10 days old) in São Paulo State, Brazil, presented positive results to suckling mice assay (fluid accumulation) but none caused cytopathic effects on Vero and CHO cells, indicating that these strains did not produced LT or VT toxins. PCR assays showed that these 27 E. coli strains harbored estA, that codifies for STa, but not for stx1, stx2 or cnf genes. The positive STa strains were checked for genes that codify for F41, F17 and K99 fimbriae, wich are considered colonization factors in ETEC. Only F17 was detect in two samples (7.4%). Twelve of 27 STa positive carried hlyA gene and presented hemolytic activity in blood Agar. Presence of rotavirus was... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Lambs; STa; Colibacillosis; Escherichia coli. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000500015 |
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| Assis,ACB; Santos,BM. |
| O objetivo desse trabalho consistiu em determinar a patogenicidade in vivo e in vitro de 25 amostras de Escherichia coli obtidas de aves com Síndrome da Cabeça Inchada (SCI). A patogenicidade in vivo foi determinada pela inoculação em pintos SPF de um dia de idade, utilizando-se quatro vias de inoculação: intracardíaca, saco aéreo, oral e traqueal. As aves foram avaliadas quanto à mortalidade a cada 12 horas, por um período de sete dias. Os resultados desse estudo demonstraram que as 25 amostras de E. coli, isoladas de aves com SCI, causaram mortalidade mais elevada em pintos de um dia de idade quando foram inoculadas pelas vias intracardíaca (73,6%) e saco aéreo (68%). As menores taxas de mortalidade em pintos de um dia de idade foram obtidas pelas vias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Colibacilose; Pintos; Síndrome da cabeça inchada; Verotoxina. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000200007 |
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| Astolfi,R.S.; Khouri,D.G.; Brandizzi,L.I.V.; Ávila-Campos,M.J.; Andrade Jr.,H.F. de. |
| Sepsis, the leading cause of death in intensive care units, is associated with overproduction of nitric oxide (NO) due to inducible NO synthase (iNOS), responsible for some of the pathologic changes. Aminoguanidine (AG) is a selective iNOS inhibitor with reported inconsistent actions in sepsis. To investigate the influence of iNOS, we studied models of acute bacterial sepsis using acute challenges with aerobic (Escherichia coli) and anaerobic (Bacteroides fragilis) bacteria in the presence of AG. Six-week-old, 23 g, male and female BALB/c and C57Bl/6j mice, in equal proportions, were inoculated (ip) with bacteria in groups of 4 animals for each dose and each experiment in the absence or presence of AG (50 mg/kg, ip, starting 24 h before challenge and daily... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Nitric oxide; Sepsis; Experimental model; Bacterial infections; Escherichia coli; Bacteroides fragilis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300006 |
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| Ávila,F.A.; Paulillo,A.C.; Schocken-Iturrino,R.P.; Lucas,F.A.; Orgaz,A.; Quintana,J.L.. |
| Foram utilizadas 99 vacas prenhes distribuídas em oito grupos que receberam os seguintes tratamentos: grupo I, com 29 vacas não vacinadas e seus bezerros que não receberam probiótico, ficando como controle; grupo II, com 10 vacas vacinadas e seus bezerros que não receberam probiótico; grupos III, IV e V, com 10 animais cada, vacas vacinadas e seus bezerros que receberam probiótico durante 5, 15 e 30 dias, respectivamente; os grupos VI, VII e VIII, com 10 animais cada, vacas não vacinadas e seus bezerros que receberam probiótico durante 5, 15 e 30 dias, respectivamente. Cada animal dos grupos vacinados recebeu duas doses vacinais contendo os pili K99 e A14 de Escherichia coli na dose de 5,0ml por via subcutânea. O probiótico contendo Ruminobacter... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Escherichia coli; Probiótico; Diarréia. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000100011 |
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| Bai,Xi; Yuan,Xianjun; Wen,Aiyou; Li,Junfeng; Bai,Yunfeng; Shao,Tao. |
| Background: Cold-active endo-1, 4-β-glucanase (EglC) can decrease energy costs and prevent product denaturation in biotechnological processes. However, the nature EglC from C. farmeri A1 showed very low activity (800 U/L). In an attempt to increase its expression level, C. farmeri EglC was expressed in Escherichia coli as an N-terminal fusion to protein S (ProS) from Myxococcus xanthus. Results: A novel expression vector, pET(ProS-EglC), was successfully constructed for the expression of C. farmeri EglC in E. coli. SDS-PAGE showed that the recombinant protein (ProS-EglC) was approximately 60 kDa. The activity of ProS-EglC was 12,400 U/L, which was considerably higher than that of the nature EglC (800 U/L). ProS-EglC was active at pH 6.5-pH 8.0,... |
| Tipo: Journal article |
Palavras-chave: Cellulose degradation; Cellulose; Cold-active enzyme; Endoglucanases; Enzymatic properties; Escherichia coli; Expression; Novel expression vector; N-terminal fusion; Protein S-tag; Recombinant protein. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600012 |
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| Barboza,L.L.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.; Fonseca,A.S.. |
| Semiconductor laser devices are readily available and practical radiation sources providing wavelength tenability and high monochromaticity. Low-intensity red and near-infrared lasers are considered safe for use in clinical applications. However, adverse effects can occur via free radical generation, and the biological effects of these lasers from unusually high fluences or high doses have not yet been evaluated. Here, we evaluated the survival, filamentation induction and morphology of Escherichia coli cells deficient in repair of oxidative DNA lesions when exposed to low-intensity red and infrared lasers at unusually high fluences. Cultures of wild-type (AB1157), endonuclease III-deficient (JW1625-1), and endonuclease IV-deficient (JW2146-1) E. coli, in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA; Escherichia coli; Filamentation; Laser. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000013 |
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| Bastos,E.M.A.F; Galbiati,C; Loureiro,E.M; Scoaris,D.O. |
| The activity of 23 samples of ethanolic brown propolis, from the State of Mato Grosso, was investigated against Escherichia coli ATCC 25922. The values of physical and chemical parameters showed significant variation among samples. The percentage of dry extract ranged from 2.6 to 27.6%. The index of oxidation varied from 3 to 519 seconds. All samples showed the percentage of wax higher than the limit preconized by the legislation, with values varying from 3.4 to 74.6%. The quantification of phenolic and flavonoid compounds, responsible for antimicrobial activity, ranged from 0.1 to 5.0 (w/w) and 0.02 to 0.66 (w/w), respectively, being that the higher the index of phenolic compounds the larger the zones of inhibition. Antibacterial activity was observed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abelha; Própolis; Atividade antibacteriana; Indicadores físico-químicos; Escherichia coli. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500032 |
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| Belloir, Jerome. |
| The coastline has many activities related to water quality such as bathing activities. Coastal catchments are known to be source of microbial input to the marine environment, their flows and runoff bringing important quantities of sediment, bacteria and other particles. Thus, a network of monitoring and modeling may be necessary to prevent economic losses such as a closure of beaches due to contamination. Hence, within the framework of project GIRAC, a modeling of river and E. coli flow in the Sainte-Anne catchment was developed. To implement the model AVSWAT on the catchment, a data collection (stream gauging, E. coli analyses) and work on GIS was necessary. The aim of this study is the hydrological and microbiological characterization of the catchment.... |
| Tipo: Text |
Palavras-chave: Bassin versant; Débits; Escherichia coli; SIG; Sainte-Anne; Catchment; River flow; Escherichia coli; GIS; Sainte-Anne. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00044/15571/12969.pdf |
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| Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Nunes,Maurício Pinto; Santana,Elsa Helena Walter de; Nero,Luís Augusto; Souza,Juliana Aparecida de. |
| Readycult - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult - LMX. The Readycult - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Readycult - LMX; X-GAL; MUG; Milk; Escherichia coli; Total coliforms. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100010 |
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| Beloti,Vanerli; Souza,Juliana Aparecida de; Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de. |
| Petrifilm EC and HS were compared to the MPN method to determine their efficacy to enumerate total coliforms and E. coli in 145 samples of water (76 water in natura, 69 drinking water). For water in natura, Petrifilm HS and EC showed good correlation with MPN method. In chlorinated water (< 20 col/100 mL and negatives) the agreement was low. For E.coli enumeration, Petrifilm EC showed a good correlation with MPN method. The results indicated that Petrifilm EC and HS are accurate to enumerate coliforms and E. coli in water when the expected counts are higher than 20/100 mL. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Water; Coliforms; Escherichia coli; Petrifilm. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400002 |
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| Bennemann,Paulo Eduardo; Bortolozzo,Fernando Pandolfo; Wentz,Ivo; Cardoso,Marisa Ribeiro de Itapema. |
| Foram utilizados 12 ejaculados, coletados de maneira asséptica, distribuídos em sete tratamentos, sendo um grupo controle e os demais inoculados com três diferentes concentrações de S. aureus ou E. coli (5 x 10(5), 5 x 10(6) e 5 x 10(7) UFC/ml). Durante 96 horas, foram avaliados a motilidade espermática, o percentual de acrossomas intactos (NAR), o pH e o número de unidades formadoras de colônia (UFC/ml) das bactérias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com exceção do tratamento com a inoculação de 5 x 10(7) UFC de E. coli/ml, não foi observado efeito significativo das bactérias sobre a motilidade espermática (p>0,05). Da mesma forma, não houve efeito significativo (p>0,05) do S. aureus ou... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sêmen; Acrossoma; Escherichia coli; Staphylococcus aureus. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200019 |
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| Registros recuperados: 237 | |
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